ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA by MilliporeSigma

Manufacturer MilliporeSigma
ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin. With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects the captured PEDF. Peroxidase-labeled streptavidin detects the biotin... Read more


<italic>ChemiKine™</italic> Pigment Epithelium Derived Factor, Sandwich ELISA by MilliporeSigma product image
ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA

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ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin.

With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects the captured PEDF. Peroxidase-labeled streptavidin detects the biotinylated mouse monoclonal antibody. After addition of the substrate and stop solution the amount of PEDF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and PEDF concentration: i.e., the higher the OD the higher the PEDF concentration in the sample.

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<italic>ChemiKine™</italic> Pigment Epithelium Derived Factor, Sandwich ELISA by MilliporeSigma product image

ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA

Manufacturer MilliporeSigma

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