AMSBIO has announced the introduction of CloneCatcher™ Gold DH5G Electrocompetent E. coli cells - a new product that exhibits extremely high electroporation efficiencies that approach the theoretical maximum of 3.4 x 1011 cfu/µg pUC19 DNA.
High Efficiency Electrocompetent Cells
Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic and epitope libraries. The limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E coli. Electroporation is one method to efficiently introduce DNA into E. coli.
CloneCatcher™ Gold cells meet the needs of scientists that are seeking to find rare clones or are building complex or metagenomic libraries. CloneCatcher™ Gold significantly outperforms all other commercially available Electrocompetent Cells when introducing ligated DNA. When the amount of input DNA is limited, CloneCatcher Gold enhances your likelihood of success.
CloneCatcher™ Gold requires less topoisomerase-loaded vector and therefore can reduce your overall operating costs. Supplied in convenient single-use tubes - CloneCatcher™ Gold can also enhance your productivity as the whole protocol for use takes only 10 minutes.