The combined Tecan/Sword offering represents the first revolution in mainstream detection technology for more than 15 years, and provides researchers with a rapid method to improve immunoassay performance in life science applications. The Sword technology extends sensitivity over current detection technologies, while being easily incorporated into assays that use a peroxidase-based detection system. Further benefits include precision and accuracy at low concentrations, allowing assay developers to improve overall assay performance, use smaller sample volumes and decrease overall time to results.
“We are pleased to be working with Sword Diagnostics on this joint launch,” said Frederic Vanderhaegen, Senior Vice President, Head BU Life Science for Tecan. “The simplicity in improving immunoassay performance that this product provides is of great importance to our customers.”
“Sword is excited to be working with an innovative and well-respected company like Tecan,” added Dave Dingott, Sword’s President and CEO. “This very high quality line of plate readers is a perfect fit for our performance enhancing reagents.”
The Infinite M1000, Tecan’s flagship modular multimode microplate reader, is equipped with premium Quad4 Monochromators™ technology and also features adjustable bandwidth settings to ultra-fine tune the amount of excitation and emission light and optimize the sensitivity for fluorescence-based measurements. The Infinite 200 PRO series provides excellent wavelength accuracy, specificity and reproducibility of monochromator-based technology. It offers the possibility to combine reagent dispensing with fluorescence, luminescence or absorbance in an affordable reader platform for the complete range of life science applications.
Sword’s technology utilizes a unique substrate that generates a light scattering, energy exchanged (Raman) signal. The principle of the Raman ELISA substrate is similar to that of colorimetric assays where a nearly colorless compound is oxidized by horseradish peroxidase (HRP) in an ELISA to form a colored reagent that is quantified by a plate reader. Sword’s substrate is a molecule with a very low Raman signal but, after oxidation by HRP and development with the Sword reagents, it has a strong and quantitative Raman signal that can be measured using the fluorescence channel of Tecan plate readers.