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New Column Toolbox Simplifying your LC Methods

Restek Corp.
27 Mar 2012
Roger Wayman
Administrator / Office Personnel

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Restek’s USLC™ column set represents a wide range of reversed phase selectivity available with just 4 stationary phases: Aqueous C18, IBD, Biphenyl, and PFP Propyl. It simplifies column choice for fast, effective method development—and the new USLC™ toolboxes make things even easier!

A USLC™ method development toolbox contains all 4 USLC™ stationary phases in 1 convenient package. Available for UHPLC (1.9 µm) and HPLC (3 or 5 µm) in 50, 100, or 150 mm lengths, this companion for method developers also includes a selection guide to help ensure that you always choose the right column the first time.

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Ultra Selective Liquid Chromatography™

Restek Corp.

Reversed Phase Method Development that's Simple, Fast, Effective, and Universal!•Boasts the widest range of reversed phase selectivity in the industry.•Simplifies column selection down to 4 unique stationary phases.•Employs orthogonal selectivity to speed up effective method development.•Maximizes efficacy of your column screening systems.•Fits any HPLC or UHPLC instrumentation. Restek Ultra Selective Liquid Chromatography™ (USLC™) technology is the directed application of selectivity—the most influential factor affecting resolution—to optimize separations and improve method performance. Restek has extensively studied reversed phase selectivity, and as a result, we offer chromatographers the most effective and widest range of USLC™ stationary phase chemistries in the industry! Pinnacle® DB columns (1.9 µm) feature base deactivated silica and are recommended for scalable UHPLC and HPLC analyses. Ultra columns (3 and 5 µm) use highest purity silica and are well suited for traditional HPLC analyses. Viva columns incorporate wide pore silica, making them ideal for large-molecule biological separations using HPLC.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.Ultraselective Column

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