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Promega GoTaq Probe Real-Time PCR Systems Provide Sensitive Quantitation with Resistant-to-Inhibitor Master Mixes

Promega Corp.
17 Oct 2012

Product news

Promega Corporation has announced the launch of the GoTaq® Probe PCR Master Mix, GoTaq Probe 1-Step RT-qPCR System, and GoTaq Probe 2-Step RT-qPCR System. These ready-to-use, 2x master mixes simplify the preparation of reactions for qPCR using hydrolysis probe detection, and are designed for sensitive sequence detection and quantification over a broad range of DNA or RNA targets, gene expression analysis, detection of sequence variants, and fluorescent detection.

Rapid hot-start activation and enzymes make GoTaq Probe Systems compatible with any real-time PCR instrument using standard or fast cycling programs. The 1-step and 2-step systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. These Systems are compatible with other probe assays such as molecular beacons.

GoTaq Probe qPCR Master Mix, designed for qPCR from gDNA templates, provides resistance to a wide range of PCR inhibitors. Using antibody-mediated hot-start chemistry, reaction set-up can be performed at room temperature. The master mix does not contain a reference dye, however carboxy-X-rhodamine is included with the system, to be added to amplification reactions if desired.

GoTaq Probe 1-Step RT-qPCR System combines GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in single-step, real-time amplification reactions for the detection and relative quantification of RNA expression levels using a one-step RT-qPCR method.

GoTaq Probe 2-Step RT-qPCR System enables detection and relative quantification of RNA expression levels via a two-step RT-qPCR method, using the integrated components of the GoScript Reverse Transcription System and GoTaq Probe qPCR Master Mix. GoScript Reverse Transcription System includes an optimized reaction buffer and reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product may be added directly to downstream qPCR amplification reactions.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Gene ExpressionRT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Promega GoTaq Probe Real-Time PCR Systems Provide Sensitive Quantitation with Resistant-to-Inhibitor Master Mixes