New LudgerTag™ 2AB and 2AA Glycan Labeling Kits with Non Toxic Reductant
05 Dec 2012

Fluorescent labeling methods for glycoprofiling of therapeutic glycoproteins are well established and aid subsequent separation and quantitation using a range of techniques. These include high performance liquid chromatography (HPLC and UHPLC), capillary electrophoresis (CE), and mass spectrometry. Of the fluorescent labels available, 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) are most widely used for oligosaccharide profiling. Existing 2-AA and 2-AB labeling kits use sodium cyanoborohydride as a reducing agent during glycan labeling. This reagent is toxic.

Following on from research performed by Ludger Ltd. in collaboration with Leiden University Medical Centre, The Netherlands, we have developed labeling kits containing a different and non-toxic reducing agent, 2-picoline borane (2-PB). This technology has now been patented and exclusively licensed to Ludger Ltd. We are delighted to offer two new kits for labeling glycans with either 2-AB or 2-AA.

The kits have been validated following ICH Q2(R1) guidelines and beta-tested in the field. Precision values were excellent, with CVs of <5% for peaks with relative areas over 5% and CVs of <8% for peak with relative areas less than 5%. The kits offer equivalent labeling efficiency as standard LudgerTag™ 2AA and 2AB labeling kits containing sodium cyanoborohydride.

Each kit comprises two premixed bottles; one containing acetic acid and DMSO solution and the other containing 2-AB or 2-AA label combined with 2PB. This makes the labeling process simpler.

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Ludger Ltd.