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| ChIP protocol to rapidly characterize DNA-protein interactions 25 Jun 2009: In the study of DNA-protein interactions, chromatin immunoprecipitation (ChIP) has emerged as a powerful technique for discovery and characterization of protein-mediated transcriptional regulation. A method is described which gives researchers a means to rapidly characterize highly abundant DNA-protein interactions, providing a relatively easy way to perform kinetic or conditional analyses for polymerase- or modified histone-DNA interactions. Sigma-Aldrich Corporation |
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| Workflow for Determining the Apoptotic Effects of Caspase-8 Overexpression in HeLa Cells 24 Jun 2009: The activation of caspases is an important process in apoptosis, for example activated caspase-8 leads to the cleavage and subsequent activation of the effector caspase, caspase-3. Using FuGENE® HD Transfection Reagent, the Cell Proliferation Reagent WST-1 and the xCELLigence System in a workflow involving both in vitro and in vivo assays it is shown that overexpression of caspase-8 alone is sufficient to induce apoptosis in HeLa cells. Roche Applied Science - a member of the Roche Group |
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| Saving Green by Going Green: Foreseeing the Future Beyond the Acetonitrile Shortage 24 Jun 2009: Acetonitrile is the chromatographers organic solvent of choice in HPLC. However in recent times, the demand for the limited volume of acetonitrile has driven the price to new highs making HPLC involved research very expensive. In this article, use of supercritical fluid chromatography (SFC) is examined not only as an alternative in eliminating the need for acetonitrile and reducing expenses, but also show its ability to improve lab efficiency and productivity. JASCO (USA) |
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| Biomarker Discovery using Quantitative Methylation-Specific PCR (qMSP) 23 Jun 2009: The OpenArray® Real-Time qPCR platform from BioTrove delivers quantitative methylationspecific PCR. This application note finds the correlations between methylation status and clinicopathological status. With one flexible tool you can identify genes inactivated by hypermethylation, and quickly switch to a screening protocol to group 100’s of samples a day based on a gene methylation profile. BioTrove |
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| Determination and Confirmation of Priority Pesticide Residues in Baby Food 22 Jun 2009: Growth in the use of pesticides, along with poor agricultural practise and illegal use, can pose significant human health risks due to the presence of pesticide and metabolite residues in food products. In order to meet imposed pesticide regulations sensitive analytical techniques are required to detect residue levels. Here Waters® utilise their UPLC® system combined with the Xevo™ TQ Mass Spectrometer for rapid determination and confirmation of pesticide residues in baby food. Waters |
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| Optimized cell dispensing with the Thermo Scientific Multidrop Combi reagent dispenser 16 Jun 2009: High throughput cell-based assays are an important part of drug screening and successful dispensensing of viable cells is one of the key issues for HTS. The peristaltic pump technology of the ThermoScientific Multidrop Combi assures a fast and gentle way of dispensing cells on any plate and well size. Cells remain intact and viable, and the plate can be used for any cell-based assay needed. Thermo Fisher Scientific |
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| Applying the Technology of the TurboMatrix 650 ATD to the Analysis of Liquid Accelerants in Arson Investigation 15 Jun 2009: As with all forensic analyses, the data generated in arson investigation must be legally defensible, creating the need for duplicate analyses and sample archival. This study discusses how the use of solvents for sample preparation can be eliminated and how sample recollection can be implemented on the TurboMatrix™ 650 ATD, using thermal extraction combined with dynamic headspace and automated thermal desorption. PerkinElmer, Inc. |
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| Analysis of Explosives and Firearms Residue Using Thermo Scientific Atlas CDS™ at Forensic Science Northern Ireland 12 Jun 2009: Illegal use of firearms and explosives materials by criminal or terrorist organizations and other groups or individuals is a growing global concern. One of the challenges facing many forensic science institutions is the detection of trace amounts of firearms and explosives residue on the hands, face, hair and clothing of suspects, or their surroundings. The installation of Thermo Scientific Atlas CDS at FSNI has allowed the laboratory to continue to develop its services in this specialist field. Thermo Fisher Scientific |
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Streamlining Cell Culture Liquid Handling 11 Jun 2009: Successful manipulation of cell cultures relies on the ability to maintain sterility and to avoid cross-contamination between cell lines. Proficiency in cell culturing can be greatly enhanced by a highly controlled workflow and a well-organised work area. A new application note describes the implementation, within a life science organisation, of a new Liquid Handling Set developed to support safe, streamlined and organised working when pipetting and aspirating cell culture media.INTEGRA Biosciences AG |
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