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| Live Cell Imaging of Drug Effects on Golgi Morpholgy Using the CELLview™ Cell Culture Dish 01 Mar 2010: This application note by Greiner Bio-One shows that performing live-cell imaging experiments in parallel in CELLview™ dishes with four compartments allows a direct comparison of the speed and timing of drug effects on the Golgi apparatus. GalNAc-GFP cells can be easily imaged at multiple positions without cell death or focus problems. Greiner Bio-One GmbH |
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| 3D-CellHOST – Convenient High Cellomics Yield Cell Culture 01 Mar 2010: This application note demonstrates the integration of the BioLevitatorTM benchtop cell culture device into Hamilton’s liquid handling platform. Four independent and high density cultures were grown in parallel, achieving a high cell yield on a small footprint. HAMILTON Life Science Robotics |
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| BD PureCoat Surfaces for Improved Cell Attachment 01 Mar 2010: This application note by BD Biosciences demonstrates enhanced cell attachment and growth in serum-reduced or serum-free culture media using BD PureCoat surfaces. Improved freeze-thaw recovery of cryopreserved cells is also achieved and cells remain functional in cytoxicity and transfection assays. BD Biosciences |
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| Determination of Well-to-Well Crosstalk in Microplates used for Luminescence Measurements 01 Mar 2010: This application note evaluates well-to-well crosstalk in commercially available microplates. The combination of effective quenching by carbon black and increased reflectivity from titanium dioxide brightener yields an improved signal-to-noise ratio and better intra-plate dynamic range, giving screeners the opportunity to screen for weaker hits at lower detection levels. Porvair Sciences Ltd |
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| Protein/Drug Interaction: Ferulic Acid and Bovine Serum Albumin 22 Feb 2010: SPR has been demonstrated to be a powerful optical technique for bioaffinity studies at the solid/solution interface. This application note describes the kinetic studies of ferulic acid and bovine serum albumin (BSA) pre-immobilized onto an alkanethiol self-assembled monolayer (SAM)-modified gold sensor chips. Biosensing Instrument |
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| HTRF® Human Interleukin beta (IL1β) Assay 15 Feb 2010: This technical note describes the successful implementation of HTRF® measurements on the Tecan InfiniteTM F500 filter based multifunctional reader. The InfiniteTM F500 was proven to be a very well-suited detection platform regarding sensitivity and dynamic range of the investigated IL-1β detection - HTRF®assay kit. Cisbio Bioassays |
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| Functional Screening of Recombinant CHO-M1 Cells using the PHERAstar from BMGLABTECH 09 Feb 2010: This application note demonstrates the HTRF® assay from Cisbio as a fast and reliable method to directly screen a large number of stable transfected single cell clones. Carbachol dose response curves with fresh cells from a growing culture and frozen instant cells measured on BMG LABTECH´s PHERAstar showed similar results for EC50 and signal-to-blank values. BMG LABTECH |
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| Establishing a Cell Culture Assay Based on Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) for Screening G-Protein Coupled Receptors 09 Feb 2010: Time-resolved fluorescence resonance energy transfer (TR-FRET) has become a popular technique in the field of high-throughput screening. This application note demonstrates the TR-FRET kit from Cisbio, the IP-One HTRF assay, under standard high-throughput screening conditions. The IP-One HTRF assay was shown to be a stable cell culture time-resolved fluorescence assay for screening G-Protein coupled receptors. Greiner Bio-One GmbH |
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| Tag-lite®: New HTRF® Cellular Platform for Cell Surface Receptors’ Study and Screening 09 Feb 2010: In a recent publication, Maurel et al presented the combination of Cisbio’s HTRF and SNAP-and CLIP- Tag technologies to investigate cell-surface protein-protein interaction. In this study, an application for GPCR oligomerization was accurately documented and the advantages of using the Cisbio HTRF-based cell-surface receptor platform presented. Applications such as highly selective and non-radioactive ligand binding assays are described and the 7TM dimerization model can properly be inv Cisbio Bioassays |
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BD Biosciences Launches New Cell Analyzer for Complex Multicolor Experiments in Disease and Drug Development Research 
