Phenyl Membrane Adsorber for Bioprocessing
3 May 2011

In this application note by Sartorius, conditions are optimised for the use of chromatographic membranes in large scale capturing and impurity removal. The low substitution of the phenyl ligand on the membrane allows for mild elution of biomolecules whilst preserving their biological functions. The influence of flow rate, salt type and concentration on the protein binding capability of phenyl membrane are analysed. In addition, aggregate removal, monoclonal antibody binding and separation of cytochrome c, myoglobin, lysozyme and α-chymotrypsinogen are assessed.

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