"The Antibody Trilogy"Cambridge Healthtech Institute

News

"The Antibody Trilogy"
11 Apr 2006

Problems with humanization, folding, expression levels, immunogenicity and cost continue to plague the field. Don't miss this opportunity to attend a Trilogy of meetings devoted to the expression of difficult proteins, including: 2 full days of problem solving for "finicky" proteins (Difficult to Express Proteins), 2 full days devoted specifically to expressing and engineering Recombinant Antibodies and a full day devoted to Monoclonal Antibodies.

Besides an excellent array of scientific presentations, you will have numerous opportunities to network with other researchers working on similar issues.

SESSIONS & PRESENTATIONS

BASIC SCIENCE FOR "FINICKY" PROTEINS

Process Conditions which Control Solubility and Proteolysis
Impact of Native State Stability on Protein Secretion Levels
Synthetic Peptide Scaffolds for Improving Bioproduction Microenvironments
Solution by Fusion: A Parallel Approach to Address Problems with Proteins
Effects of Truncation on Protein Expression and Crystallization

EXPRESSION, PURIFICATION AND CHARACTERIZATION SCIENCE AND STRATEGIES

Split GFP and Its Application to Trapping Soluble Protein Domains
Application of a System for Rapid Evaluation of Recombinant Protein Expression in Insect Cells
High-Throughput Protein Production and Cell-Based Screening of a Comprehensive Secreted Protein Library
Applications of the Wheat Germ Cell-Free Technology in Automated High-Throughput Production of Eukaryotic Proteins

CASE STUDIES

Smart Solutions for the Overexpression of Soluble Mammalian Protein Targets
Expression of Functional Peripheral Cannabinoid Receptor for Structural Studies
Development of Filamentous Fungi as Industrially Relevant Gene Expression Hosts: Expression of the Thermophilic Enzyme Xylanase B (XynB) in Trichoderma reesei
Exceptional Total and Functional Yields of the Human Adenosine (A2a) Receptor Expressed in the Yeast Saccharomyces Cerevisiae
The Use of Cell-Free Systems to Produce Proteins for Functional and Structural Applications
Pressure-Enhanced Protein Aggregate Refolding: Advantages in Comparison to Traditional Chaotrope-Based Methods
Protecting and Stabilizing Proteins against Aggregation to Ease Their Processing and Production

NOVEL APPLICATION OF EXISTING PLATFORMS

Baculovirus Expression of Two Taeniasis Diagnostic Antigens in Insect CellsAn Automated Micro-Fermenter Battery for Producing Recombinant Proteins
An Improved S. cerevisiae System for Rapid and Inexpensive Production of Recombinant Proteins
Information Rich Design of Expression Optimized Synthetic Genes

KEYNOTE PRESENTATION

Affinity Maturation of Antibodies: Insights from the Physiological Mechanism

ALTERNATIVE NATURAL IMMUNE RECEPTORS

Structural and Functional Analysis of Hypervariable Immune Receptors in Insects
Leucine-Rich Repeat (LRR) Modules: Linking Innate and Adaptive Immunity
Comparative Studies of Immunoglobulins with an Emphasis on Single-Chain Antibodies in Sharks
Nanobodies: The Smallest Intact Antigen-Binding Fragments Derived from Camel Heavy Chain Antibodies
ANTICALINS: Small Antibody-Like Binding Proteins, Derived from the Lipocalin Scaffold, for Therapeutic Applications

STRATEGIES FOR IMPROVING ANTIBODY SELECTION, EFFICACY AND PRODUCTION

Production of Recombinant Antibodies for Low-Abundance Proteins
Cloned, Transgenic Livestock Produce Bispecific Antibodies for Immunotherapy of Melanoma
Finding Rare and Unique Antibodies using Multiple Phage Display Libraries
A Novel Non-Display Epitope Focused Library Method for Making 'Humaneered' Antibodies
Rapid Custom Generation of Recombinant Antibodies

APPLICATIONS: Toxins, Biothreats and Emerging Diseases

KEYNOTE ADDRESS - The Botulinum Antitoxin Story
Passive Immunotherapy in an Outbreak Setting. A Case of SARS Antibodies.
Human mABs for the Prophylaxis of West Nile Virus Infection
Antibody-Based Therapeutics for HIV-1, Emerging and Biodefense-Related Viruses and IGF-Dependent Cancer

ASSAYS AND MONITORING

Monitoring Product Quality as an Integral Part of Cell Line Development Process
From DNA Array to Protein Array
Mass Spectrometric Analysis of Intact Antibodies and Immunoconjugates

SELECTION, CHARACTERIZATION AND PRODUCTION

High-Throughput Strategies for Monoclonal Antibody Selection and Production Cell Line Isolation
Engineering Nanobodies for Therapy: Dealing with Bivalency, Bispecificity and Potency Optimization
Increased Production of Humanized Monoclonal Antibodies in Chinese Hamster Ovary Cells
Peptide Constructs for Immunization Against 'Impossible' Epitopes
Rapid Cell Line Enrichment

ENHANCING EXPRESSION, SAFETY, AND EFFICACY

Immunoassay Format Effects on Immunogenicity Assessment for Monoclonal Antibody Therapeutics Antibody Humanization by Framework Shuffling
Measurement of the Functional Affinity Constant of a Monoclonal Antibody for Cell Surface Receptors
using Kinetic Exclusion Fluorescence Immunoassay

CASE STUDIES