"The Antibody Trilogy"

11 Apr 2006
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Problems with humanization, folding, expression levels, immunogenicity and cost continue to plague the field. Don't miss this opportunity to attend a Trilogy of meetings devoted to the expression of difficult proteins, including: 2 full days of problem solving for "finicky" proteins (Difficult to Express Proteins), 2 full days devoted specifically to expressing and engineering Recombinant Antibodies and a full day devoted to Monoclonal Antibodies. 

Besides an excellent array of scientific presentations, you will have numerous opportunities to network with other researchers working on similar issues.

SESSIONS & PRESENTATIONS

BASIC SCIENCE FOR "FINICKY" PROTEINS

  • Process Conditions which Control Solubility and Proteolysis
  • Impact of Native State Stability on Protein Secretion Levels
  • Synthetic Peptide Scaffolds for Improving Bioproduction Microenvironments
  • Solution by Fusion: A Parallel Approach to Address Problems with Proteins
  • Effects of Truncation on Protein Expression and Crystallization

EXPRESSION, PURIFICATION AND CHARACTERIZATION SCIENCE AND STRATEGIES

  • Split GFP and Its Application to Trapping Soluble Protein Domains
  • Application of a System for Rapid Evaluation of Recombinant Protein Expression in Insect Cells
  • High-Throughput Protein Production and Cell-Based Screening of a Comprehensive Secreted Protein Library
  • Applications of the Wheat Germ Cell-Free Technology in Automated High-Throughput Production of Eukaryotic Proteins

CASE STUDIES

  • Smart Solutions for the Overexpression of Soluble Mammalian Protein Targets
  • Expression of Functional Peripheral Cannabinoid Receptor for Structural Studies
  • Development of Filamentous Fungi as Industrially Relevant Gene Expression Hosts: Expression of the Thermophilic Enzyme Xylanase B (XynB) in Trichoderma reesei
  • Exceptional Total and Functional Yields of the Human Adenosine (A2a) Receptor Expressed in the Yeast Saccharomyces Cerevisiae
  • The Use of Cell-Free Systems to Produce Proteins for Functional and Structural Applications
  • Pressure-Enhanced Protein Aggregate Refolding: Advantages in Comparison to Traditional Chaotrope-Based Methods
  • Protecting and Stabilizing Proteins against Aggregation to Ease Their Processing and Production

NOVEL APPLICATION OF EXISTING PLATFORMS

  • Baculovirus Expression of Two Taeniasis Diagnostic Antigens in Insect CellsAn Automated Micro-Fermenter Battery for Producing Recombinant Proteins
  • An Improved S. cerevisiae System for Rapid and Inexpensive Production of Recombinant Proteins
  • Information Rich Design of Expression Optimized Synthetic Genes

KEYNOTE PRESENTATION

Affinity Maturation of Antibodies: Insights from the Physiological Mechanism

ALTERNATIVE NATURAL IMMUNE RECEPTORS

  • Structural and Functional Analysis of Hypervariable Immune Receptors in Insects
  • Leucine-Rich Repeat (LRR) Modules: Linking Innate and Adaptive Immunity
  • Comparative Studies of Immunoglobulins with an Emphasis on Single-Chain Antibodies in Sharks
  • Nanobodies: The Smallest Intact Antigen-Binding Fragments Derived from Camel Heavy Chain Antibodies
  • ANTICALINS: Small Antibody-Like Binding Proteins, Derived from the Lipocalin Scaffold, for Therapeutic Applications

STRATEGIES FOR IMPROVING ANTIBODY SELECTION, EFFICACY AND PRODUCTION

  • Production of Recombinant Antibodies for Low-Abundance Proteins
  • Cloned, Transgenic Livestock Produce Bispecific Antibodies for Immunotherapy of Melanoma
  • Finding Rare and Unique Antibodies using Multiple Phage Display Libraries
  • A Novel Non-Display Epitope Focused Library Method for Making 'Humaneered' Antibodies
  • Rapid Custom Generation of Recombinant Antibodies

APPLICATIONS: Toxins, Biothreats and Emerging Diseases

  • KEYNOTE ADDRESS - The Botulinum Antitoxin Story
  • Passive Immunotherapy in an Outbreak Setting. A Case of SARS Antibodies.
  • Human mABs for the Prophylaxis of West Nile Virus Infection
  • Antibody-Based Therapeutics for HIV-1, Emerging and Biodefense-Related Viruses and IGF-Dependent Cancer

ASSAYS AND MONITORING

  • Monitoring Product Quality as an Integral Part of Cell Line Development Process
  • From DNA Array to Protein Array
  • Mass Spectrometric Analysis of Intact Antibodies and Immunoconjugates

SELECTION, CHARACTERIZATION AND PRODUCTION

  • High-Throughput Strategies for Monoclonal Antibody Selection and Production Cell Line Isolation
  • Engineering Nanobodies for Therapy: Dealing with Bivalency, Bispecificity and Potency Optimization
  • Increased Production of Humanized Monoclonal Antibodies in Chinese Hamster Ovary Cells
  • Peptide Constructs for Immunization Against 'Impossible' Epitopes
  • Rapid Cell Line Enrichment

ENHANCING EXPRESSION, SAFETY, AND EFFICACY

  • Immunoassay Format Effects on Immunogenicity Assessment for Monoclonal Antibody Therapeutics Antibody Humanization by Framework Shuffling
  • Measurement of the Functional Affinity Constant of a Monoclonal Antibody for Cell Surface Receptors 
    using Kinetic Exclusion Fluorescence Immunoassay

CASE STUDIES

  • Isolation of Neutralizing Monoclonal Antibodies to Toxin A and Toxin B From Clostridium Difficile
  • Tie-1 Targeted Inhibitor of Tumor Growth

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Cambridge Healthtech Institute