Optimization of Trypsin Digestion for MRM Quantification of Therapeutic Proteins in Serum
19 Feb 2013

In this application note a general workflow for the quantification of therapeutic proteins in serum without analyte pre-fractionation was tested to determine the quantification of trastuzumab in human serum. The analytical workflow of trypsin digestion can be optimized to develop a successful multiple reaction monitoring (MRM) assay. Some of the benefits of trypsin digestion include higher assay sensitivity, reduced sample preparation time, and higher assay reproducibility.

Popular tags in SelectScience

Buying guides

Write a Review

Join the Global SelectScience Community Today! It’s FREE!

Helping you make informed decisions about the latest lab products and technologies:

  • Be the first to learn about new technologies
  • 10,000+ trusted product reviews from your peers
  • Compare 500,000 products and manufacturers
  • Get exclusive buying tips from experts
  • Find solutions fast: 10,000+ Application Notes, Webinars and Videos

Connect with 250,000 scientists... All in one place!

Become a member today!