Analysis and Purification of Diastereomeric Steroid Saponins from the Trigonella foenum-graecum Seed Extract

This application note reports the chromatographic resolution of a pair of 25 R/S spirostanol saponin diastereomers from Trigonella foenum-graecum seed extract using the Waters Method Station SFC System. The advantages of using SFC for spirostanol saponin analysis and purification are discussed.