Application Note: Direct PCR from Blood Preserved on Whatman FTA and 903 Cards using Thermo Scientific Phusion Blood Direct PCR Kit
6 Feb 2014
Paper cards are a popular method of storing blood due to their ease of use and long term stability at room temperature. However amplification of DNA directly from storage cards samples is particularly challenging due to PCR inhibitors present. With conventional DNA polymerases, a thorough washing protocol is required. This study demonstrates that Phusion Blood Direct PCR Kit allows amplification of DNA directly from blood stored on cards with no or very little pretreatment.