ResourceLife Sciences
Epigeneous™ H3K27Me3 Cellular Assay for Measuring Epigenetic Methylation
7 Sept 2014This application note demonstrates that these cell-based assays were optimized to meet the format requirements of researchers screening epigenetic targets or using them as phenotypic read-out of upstream cell targets. For high throughput screening purposes, the no-wash streamlined assay protocol needs a robust liquid handling system in order to produce the most reliable output. From that perspective, this paper illustrates very clearly how HTRF assays can be easily implemented on the Fluent laboratory automation solution to offer an efficient and robust high throughput screening solution.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Epigenetic AnalysisEpigenetic analysis products are used to study heritable phenotype changes that do not alter the DNA sequence, such as DNA modifications affecting gene activity. Determine whether genes have been methylated with DNA methylation quantification kits or methylation microarrays. Assess epigenetic regulation with histone modification kits or ChIP-on-chip microarrays. Kits are also available for bisulfite conversion and amplification of your samples, as well as epigenomic library preparation kits for next-generation sequencing (NGS). Find the best epigenetic products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.MethylationHTRFScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.
