Odyssey® In-Gel Western Detection Using NIR Fluorescence

12 Jun 2018

The Odyssey® Infrared Imaging system allows you to detect target proteins while still embedded in the gel using near-infrared secondary antibodies. Using near-infrared fluorescence detection methods for In-Gel Westerns makes this a powerful technique. Here, a protocol for In Gel Western analysis and tips for its optimisation is presented.

Odyssey DLx Infrared Imaging System

LICORbio

The Odyssey DLx Imaging System delivers accurate, reproducible, and quantitative data with near-infrared fluorescence. It supports Western blots, In-Cell Western™ Assays and other plate-based assays, cell-based assays, EMSAs, DNA and protein gels, protein arrays, and tissue section imaging. Discover more with the groundbreaking Odyssey technology cited in over 120,000 peer-reviewed publications.

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Odyssey M Imaging System

LICORbio

The Odyssey M is the most versatile and sophisticated biomolecular imager on the market. With 19 available channels, industry-leading 5 µm resolution, >6 logs of dynamic range, and an onboard luminescence module, the Odyssey M gives researchers a multimodal imaging solution with unparalleled capabilities and performance. Discover more with the groundbreaking Odyssey technology cited in over 40,000 peer-reviewed publications.

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Odyssey® In-Gel Western Detection Using NIR Fluorescence