Application Note: Odyssey® In-Gel Western Detection Using NIR Fluorescence
13 Jun 2011
The Odyssey® Infrared Imaging system allows you to detect target proteins while still embedded in the gel using near-infrared secondary antibodies. Using near-infrared fluorescence detection methods for In-Gel Westerns makes this a powerful technique. Here, a protocol for In Gel Western analysis and tips for its optimisation is presented.