Application Note: High-Fidelity Amplification of GC-Rich DNA is a Challenging Application Due to the Low Processivity and Lack of Robustness of Most Proofreading DNA Polymerases
1 September 2014
This application note demonstrates the novel KAPA HiFi DNA Polymerase and its proprietary buffer system, which are ideally suited for the high-fidelity amplification of DNA with a GC content >65%. The unique properties of this enzyme include high processivity and increased tolerance to DNA melting agents, which enables robust, amplification of GC-rich DNA with extremely low error rates.