Application Note: Co-Culture in ThinCert Cell Culture Inserts
2 October 2012

Co-cultures with cell culture inserts are widely used to study mesenchymal-epithelial interactions during normal and tumoral development. In this application note such a co-culture model using MCF7 mammary carcinoma cells, human fibroblasts and ThinCert™ cell culture inserts from Greiner Bio-One GmbH is described. The results show that the use of ThinCert™ cell culture inserts with small pores (0.4 µm) guarantees that primarily paracrine signaling and not direct cell-cell contact accounts for the observed effects.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me