ResourceForensics

Evaluation of Evaporative Sample Preparation Techniques for Alcohol Markers and Drugs of Abuse in Hair Samples

30 Apr 2012

Read this case study and learn how the analysis of hair can be a useful tool in many forensic and clinical applications to establish drug use, trends of use and in the assessment of chronic alcohol consumption. Using the Genevac evaporation programme within these DoA produces, enables the excellent recovery of amphetamine, for example, which is renowned for its volatility.

EZ-2 Series

Genevac

Using the most advanced proven technology in evaporation science, the third generation of the EZ-2 Series has been designed specifically for solvent removal in life science research; be that concentration of samples or complete drying.  The innovative design of the EZ-2 Series presents many advantages for working in parallel within synthesis chemistry, analytical sample preparation and for general-purpose solvent removal needs in life science laboratories. At the heart of the evaporator is a solvent resistant, oil-free vacuum pump, and the high efficiency, defrost-free, SpeedTrap™, which traps all solvents as liquids, even water. Solvents collect in the insulated, plastic coated glass vessel, enabling the user to see the progress of evaporation and determine when the trap should be emptied. The EZ-2 Elite has a special auto-draining collection vessel fitted to the SpeedTrap, which uses the very powerful Scroll pump. The Elite can therefore evaporate the highest boiling solvents such as DMSO and perform the Genevac LyoSpeed fast lyophilisation method for HPLC fractions. There is a low-cost option available on the Standard, Plus and Elite that allows the routine use of hydrochloric acid and aggressive solvents. By building key elements in Hastelloy®, glass or PTFE, full protection against acid chlorides is achieved. New to the HCl Resistance option is the ability to evaporate concentrated nitric acid. This option must be specified when ordering, as it cannot be retrofitted. All EZ-2 models are resistant to routine use of high concentrations of TFA as standard. An additional option is available to allow safe operation for those wishing to use highly explosive solvents, such as pentane or diethyl ether - the inert gas purge system. This system can also be used to preserve oxygen or moisture sensitive samples under an inert gas blanket until the user returns to the evaporator. The EZ-2 Series can also accommodate a wide selection of sample holders, including round-bottom flasks up to 500ml, tubes up to 160mm long (including Dionex ASE® and Radleys Carousel tubes), custom reaction blocks, and shallow or deep-well microtitre plates. EZ-2 Series Models: The Standard (EZ-2) is suitable for water and volatile solvents The Envi (EZ-2 Envi) is recommended for concentration of volatile solvents The Plus (EZ-2 Plus) is recommended for solvents with boiling points upto 165°C The Elite (EZ-2 Elite) is recommended for solvents with boiling points upto 220°C

(4)

Links

Tags

LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Evidence Collection and AnalysisEvidence Collection and Analysis is the retrieval and subsequent investigation into criminal evidence obtained from a crime scene. Forensic genetic instruments, kits and reagents enable the analysis of evidence collected  at the crime scene. Reagents and kits for presumptive analysis for body fluids such as blood, saliva and semen in addition to drugs and narcotics test kits are used for CSI. Fingerprinting equipment, forensic light sources, casting kits and equipment for collecting recoverable materials can be found in the directory.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.Drugs of AbuseA drug of abuse is a drug that has been taken for non-medical purposes. Such drugs can have mind-altering effects, with some causing dependence and addiction.Anti-Doping ScienceAnti-doping science involves the detection of performance-enhancing drugs (PEDs) and banned substances in athletes. This field helps ensure fair competition and athlete safety. Explore anti-doping science tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Amphetamine
Evaluation of Evaporative Sample Preparation Techniques for Alcohol Markers and Drugs of Abuse in Hair Samples