Real-Time Monitoring of Genetically Encoded Redox Probes in Mammalian Cell Monolayers

19 Aug 2014

This application note demonstrates that the PHERAstar FS reader enables roGFP2-based measurements in mammalian cell monolayers grown in 96-well imaging plates.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.