Use of the PROTEAN i12 IEF System for In-Gel Peptide Fractionation Prior to LC-MS and Comparison with Off-Gel Fractionation
9 Oct 2012

The separation of proteolytically digested samples by LC-MS is a powerful and widely used technique for proteomic analysis. In this application note, off-gel and in-gel methods for peptide separation using IPG strips are compared. The PROTEAN® i12™ IEF System, from Bio-Rad Laboratories Inc., used for in-gel fractionations provided a greater yield of peptide identifications compared to off-gel methods used. Other benefits of in-gel fractionation are also described.

Popular tags in SelectScience

Buying guides

Write a Review

Join the Global SelectScience Community Today! It’s FREE!

Helping you make informed decisions about the latest lab products and technologies:

  • Be the first to learn about new technologies
  • 10,000+ trusted product reviews from your peers
  • Compare 500,000 products and manufacturers
  • Get exclusive buying tips from experts
  • Find solutions fast: 10,000+ Application Notes, Webinars and Videos

Connect with 250,000 scientists... All in one place!

Become a member today!