Microaerobic fermentation within gut physiological conditions

29 Jan 2020

There is growing interest across the food and feed and biofuel industries in microaerobic fermentation, a process occurring at close to anaerobic conditions, but still requiring small amounts of oxygen. This application note describes the microaerobic fermentation of a probiotic strain, Lactobacillus acidophilus, at a very low oxygen level using BioFlo® 120 and 320 control stations.

Related products

Request Quote for All Products

BioBLU® 0.3 Single-Use Vessels
Eppendorf
As the smallest vessel of the Eppendorf single-use bioreactor family the BioBLU 0.3 is ideally suited for use as a scale-down model. Designed for use with the DASbox Mini Bioreactor System it is able to accelerate process development in biopharmaceutical and biotechnological applications using cell cultures and microorganisms.All critical process parameters can be precisely controlled and users benefit from easy handling, minimized set-up time and the elimination of cleaning and sterilization steps. The unique liquid-free exhaust gas condenser reduces negative effects caused by change of medium osmolarity and efficiently prevents blocking of the exhaust gas filter.BioBLU® 0.3 Single-Use Vessels Features: Fully instrumented single-use mini bioreactors for accelerated process development in cell culture and microbial applications. Excellent scalability and reproducibility. Small working volumes starting at 65 mL save on cell material, media and supplements. Used with the DASbox mini bioreactor system for parallel set-up of 4, 8, 12 or more bioreactors. Liquid-free exhaust condenser with easy handling by automatic click in activation and click out deactivation mode. Magnet-coupled overhead drive reduces the risk of contamination, up- or downflow. Headplate with 2x PG 13.5 ports, 4x integrated long dip tubes, 2x integrated short dip tubes, one thermowell, DO sensor port with permeable membrane. Operating with industry standard probes for precise measurement and control of temperature, pH, DO. Vessels are delivered pre-sterilized by ß-irradiation to dose exceeding 15 kGy (DIN EN ISO 11137-2:2007). All wetted materials are USP Class VI certified. Applications: Process development in cell culture and microbiology Design of Experiments (DoE) Media optimization Clone & cell line screening, strain characterization
(0)
Eppendorf BioFlo® 320
Eppendorf
The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition
(2)
Eppendorf BioFlo® 120
Eppendorf
The Eppendorf BioFlo 120 offers simplicity and ease of use, without sacrificing capability. In academic, governmental or industrial research settings; for work with bacteria, yeast, fungi, mammalian, insect or plant cells: The BioFlo 120 is an attractive solution to meet bioprocessing needs. The BioFlo 120 is a bench-scale bioreactor/fermentor system that perfectly suits research and development. Its flexible design meets the wide-ranging needs of scientists in both microbial and cell culture applications. 24 interchangeable autoclavable vessels (water-jacketed or with heat blanket) are available, along with BioBLU® single-use bioreactors ranging from 250 mL to 40 L working volume. Digital Mettler Toledo® ISM sensor technology is fully integrated allowing the user to switch between pH, redox, dissolved oxygen (polarographic and/or optical), and carbon dioxide monitoring without the need for additional equipment. A high-precision thermal mass flow controller with expanded range provides the capability for low flow mammalian cell culture through high-demand microbial processes on a single control station. Features: Scale-up from 250 mL to 40 L on a wide variety of autoclavable and single-use vessels New Auto culture modes for microbial and cell culture process control at the touch of a button. Universal connections for analog sensors or digital Mettler Toledo ISM sensors for increased flexibility Applications: Cultivation of mammalian, stem, insect, and plant cells Bacterial, yeast, and fungal fermentation Batch, fed-batch, continuous or perfusion
(2)
Eppendorf BioBLU® Single-use Vessels
Eppendorf
Eppendorf BioBLU Single-use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design.
(3)