Utilizing the Labcyte Echo Liquid Handler and Gateway Gene Assembly in the Generation and Transfection of a PTEN-GFP Construct into a PTEN Null PC-3 Cell Line
24 Sept 2017The study of protein function relies upon recombinant gene assembly for protein expression and functional assays. This strategy is particularly important when available information regarding function is limited. The vast amount of data generated from genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. This requires flexible and efficient procedures for generating vectors containing gene fusions, gene variants, genetic coding for protein tags and cellular targeting. Furthermore, efficient high throughput cloning and gene assembly procedures are essential for interrogating signaling pathways with multiple gene constructs. The resulting gene constructs can be analyzed in stable transgenic or in transiently expressing biological systems. This application note demonstrates an efficient and robust gene assembly method to generate a fusion protein for functional cellular assays.
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Echo® 500 Series Liquid Handlers
The Echo® liquid handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source. This product does not use tips, pin tools or nozzles—completely eliminating contact between the instrument and the liquid. The Echo liquid handler provides powerful benefits: Tipless transfer, resulting in dramatic cost savings Improved information about sample integrity and improved transfer quality through measurement of DMSO hydration level Elimination of the need for calibration through Dynamic Fluid Analysis™ technology, a patented Labcyte process Elimination of cross-contamination, carryover or leachates through non-contact transfer Accurate, precise movement of nanoliter-scale quantities, enabling assay miniaturization and further reducing costs Better results through direct dilution of compounds, an improvement over serial dilution methods Greatly simplified assay development and dilution processes through the flexibility to transfer any volume of liquid from any well to any other well