Selecting Reagents for Multicolor Flow Cytometry
22 Sep 2011
Flow cytometers that detect six, eight, and more colors have spurred the development of new fluorochromes and antibody conjugates that take advantage of these capabilities. However, choosing the optimal combination of fluorochromes for your particular antibody specificities is a complex process. This application note provides some simple guidelines to help you select reagent panels for multicolor flow cytometry, avoid rounds of trial and error, and increase your chances of a successful experiment.