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Rapid Target and Untarget Analytical Method for Alkaloids Analysis in Herbal Extracts

Thermo Fisher Scientific

Rapid Target and Untarget Analytical Method for Alkaloids Analysis in Herbal Extracts

19 Jan 2016

Alkaloids are nitrogenous organic basic compounds produced by plants, not only as waste products of metabolic processes, but also for many biological functions (e.g., pest defence, protection from photodegradation, aid in the growth of the seeds). Some of them are responsible for the beneficial effects of traditional medicines, but some may instead have the harmful effects of poisons. In particular pyrrolizidine alkaloids (PAs) have hepatotoxic, mutagenic, and carcinogenic effects and in accordance with the German Federal Institute for Risk Assessment (BfR) a daily intake limit of 0.007 μg/kg body weight (0.42 μg/60 kg adult) was identified for 1,2- unsaturated Pas. Different studies revealed clearly that some herbal preparations may contain high amounts of PAs exceeding current recommendations. This poster demonstrates the development of a method for analyzing herb extracts which enabled the identification of about 300 alkaloids, belonging to 14 chemical groups.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.MutagenesisNatural ProductsTargeted ScreeningNon-Targeted ScreeningHepatotoxicityToxicityToxicity is defined as the degree to which a substance can harm humans or animals. It is essential to study the toxicity of pharmaceutical products during the development process so that optimum dosage can be established for the administration of the drug.Carcinogens

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Rapid Target and Untarget Analytical Method for Alkaloids Analysis in Herbal Extracts