Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies
19 Oct 2014At the Icahn Institute for Genomics and Multiscale Biology at Mount Sinai in New York City, technology development expert Robert Sebra, Ph.D., sees tremendous need for long-read, high-accuracy clinical sequencing for use in microbial surveillance, detection of repeat expansions, and more. To meet that demand, he relies on Single Molecule, Real-Time (SMRT®) Sequencing from Pacific Biosciences with BluePippin™ automated DNA size selection from Sage Science. Together, these tools offer a powerful solution and industry-leading read lengths that allow Sebra and other researchers to resolve repeat elements and structural variants, rapidly close microbial genomes, and measure epigenetic marks.
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BluePippin Size Selection System
Sage ScienceThe BluePippin is a preparative electrophoresis platform for collection of size-selected DNA or protein samples. The system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries, or upstream of protein mass-spec. For DNA applications, the BluePippin features pulsed-field electrophoresis to collect high molecular weight targets, and can collect fragments (depending on gel type) between 90 bp- 50kb. For protein, the system uses SDS-agarose to collect targets between 18-200kDa. In both cases, a higher quality of sample is extracted, and more reproducibly, when compared to manual gel purification methods.