Application Note: Platelet Counting with the BD Accuri C6 Flow Cytometer
6 November 2012

Flow cytometry can rapidly identify platelets and discriminate them from debris and red blood cells (RBCs), but counting the cells commonly requires adding counting beads to the sample, or separately determining the RBC count using a hematology analyzer, and then calculating the RBC/platelet ratio. In this application note from BD Biosciences the BD Accuri™ C6 flow cytometer was used to accurately count platelets in whole human peripheral blood specimens using the RBC/platelet ratio International Reference Method (IRM) and by direct volume computation. The direct volume method requires no additional measurement of RBC or reference bead counts, and offers the added benefits of increased speed and reduced cost and complexity.