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Perfecta3D® Hanging Drop Plates Protocol: 384-Well Plate Spheroid Transfer and Harvesting

3D Biomatrix
7 Sept 2014

Spheroids can be harvested from either the top or bottom side of the Perfecta3D Hanging Drop Plate. Once harvested, the spheroids can be processed for further culturing (e.g. differentiation, invasion assay, etc.) or analysis (e.g. flow cytometry, immunofluorescence staining, histological staining, confocal microscopy, etc.). This note presents a protocol for the transfer and harvesting of spheroids from a 384- Perfecta3D Hanging Drop Plate.

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Perfecta3D Hanging Drop Plates - 384-Well

3D Biomatrix

The patent-pending Perfecta3D® Hanging Drop Plates are 96- and 384-well plates that facilitate the formation, culture, and testing of three-dimensional (3D) spheroids and stem cell aggregates without the aid of coatings or matrices. With only one spheroid forming in each well, 3D spheroids formed in Perfecta3D Hanging Drop Plates are consistent and diameter can be controlled by the number of cells seeded in each well. Media and compounds can be added or removed from the top of the plate, and cells can be added for patterned co-cultures. The well plate format means that the plates are compatible with manual or automated liquid handling equipment. Product and ordering information can be found at www.3DBiomatrix.com/products.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.

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Perfecta3D® Hanging Drop Plates Protocol: 384-Well Plate Spheroid Transfer and Harvesting