Optimization of a Label-free Localized Surface Plasmon Resonance Assay on a Spectral Scanning Microplate Reader

12 June 2012

This note describes how to perform and optimize a SoPRano measurement on two Thermo Scientific microplate spectrophotometers. Detection of the interaction of human Serum Albumin (hSA) conjugated to gold nanoparticles and anti-hSA monoclonal antibody is used as a model assay.

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