Measurement of Reactive Oxygen Species in Arabidopsis and N.benthamiana Plants
7 Dec 2012

This application note reports the measurement of transient production of reactive oxygen species (ROS) in response to pathogen attacks using a luminescence coupled assay. The generation of ROS is a typical reaction of plants in response to pathogen infections. The measurement of ROS induced by pathogen-associated molecular patterns (PAMPs) is a valuable tool to identify and characterize PAMPs, to analyze mutants with regard to their immune system and to transfer the knowledge from Arabidopsis to other plant species. The luminescence assay paired with the Berthold Mithras LB 940 multimode reader allows for a quantitative determination of ROS by performing a redox-reaction. These measurements can serve as a robust output of pathogen induced signaling in Arabidopsis as well as in N. benthamiana leaves.

Popular tags in SelectScience

Buying guides

Write a Review

Join the Global SelectScience Community Today! It’s FREE!

Helping you make informed decisions about the latest lab products and technologies:

  • Be the first to learn about new technologies
  • 10,000+ trusted product reviews from your peers
  • Compare 500,000 products and manufacturers
  • Get exclusive buying tips from experts
  • Find solutions fast: 10,000+ Application Notes, Webinars and Videos

Connect with 250,000 scientists... All in one place!

Become a member today!