Application Note: Extraction of Opiates From Human Urine Using ISOLUTE® SLE+ 96-Well Plates and Columns
16 August 2012

This application note describes the extraction of a range of opiates from human urine using ISOLUTE SLE+ supported liquid extraction plates (96-well) and columns with LC-MS/MS analysis.
This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP)) from human urine. This simplified and efficient extraction method has significant analyte recoveries ranging from 70-102% with LOQs as low as 500 pg/mL.

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