Cell Cycle Analysis Using the BD BrdU FITC Assay on the BD FACSVerse™ System

20 October 2011

This application note describes the use of the FITC BrdU flow kit and the BrdU FITC assay to quickly acquire data from Jurkat cells that have been pulsed and unpulsed with BrdU, and generate a report. In addition, a user-defined assay was created in BD FACSuite software to demonstrate the dose-dependent effects of the DNA polymerase inhibitor aphidicolin on different phases of the cell cycle and on DNA damage. Finally, a BrdU pulse-chase experiment was performed to demonstrate the turnover of the label with cell cycle progression.

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