Application Note: Being Direct: Development and Optimization of a Direct Amplification Workflow for Single-Source Samples
5 February 2013

Short Tandem Repeat (STR) multiplex PCR kits have formed the basis of forensic DNA testing for the last 15 years. Until recently, kits were generally designed to handle all types of samples as part of a single processing workflow. Although there are several advantages to this approach, these kits do not reflect the very different nature of the samples involved or the interpretation demands associated with each sample type. This technical note discusses the recent advances in extraction technology, with a particular focus on direct amplification PCR kits and their optimization. Along with the use of the NGM SElect™ and Identifiler® Direct kit to maximize the benefits of direct amplification workflows.

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