An Automated High Throughput Approach to Permeability Screening using MDCK Monolayers and Rapid LC/MS/MS Analysis
13 May 2008
A critical step in early phase drug development is the determination of oral bioavailability. Measurement of drug transport across MDCK monolayers provide a tool for estimating passive permeability of drugs and for demonstrating efflux by p-glycoprotein. To increase throughput and reduce cost there is a critical need for automated processes for method development, sample analysis and data processing. This poster highlights an approach which utilizes novel software based automation coupled with high throughput LC/MS/MS.