Application Note: A Rapid and Robust High Content Analysis Method for Detecting Protein Kinase Phosphorylation Using acumen® hci
4 February 2013

Individual signalling events have traditionally been monitored by biochemical and lysate-based assays (e.g. western blot, immunoprecipi¬tation, ELISA). However these are limited in their ability to monitor the phosphorylation of multiple proteins on a per cell basis. In this application note the ability of acumen hci to rapidly analyze and assess kinase phosphorylation in a multiplexed assay format is demonstrated. Since cell processing and labelling is performed with microplates, this technique is highly amenable to automation.

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