Quantitative Real-Time RT-PCR
26 May 2015

This application note describes how the NanoDrop 1000 is used to normalize the amount of RNA to be added to each reverse transcription reaction. As an example, the expression of U6 RNA will be analyzed by real-time PCR in 33 specimens of human liver tissue. Basic principles of cDNA synthesis, RNA extraction, and PCR will not be described.