Master PFAS analysis for a healthier and safer future

Per-and polyfluoroalkyl substances (PFAS) pose a significant challenge in contemporary environmental and health research due to their extensive use and enduring nature. Accurate, comprehensive analysis is essential to understand the environmental implications and potential health risks linked to PFAS compounds.

From nonstick cookware to firefighting foam, PFAS have stood as cornerstone chemicals in industry and manufacturing for over 70 years. These enduring synthetic compounds, often referred to as 'forever chemicals', are resistant to breakdown and persist extensively in both the environment and the human body. Analyzing PFAS is crucial for assessing their presence and concentration in different mediums, particularly in drinking water, wastewater, and biofluids, to mitigate potential health risks associated with exposure.

Welcome to our new platform dedicated to scientists engaged in the critical field of PFAS analysis. This central hub contains knowledge and resources tailored to professionals focusing on PFAS analysis in drinking water, wastewater, and biofluids. Click below to explore, engage, and advance your expertise in PFAS analysis with us.

Drinking water

Understanding the presence and concentration of PFAS in one of our most vital resources, drinking water, is paramount for ensuring the safety and well-being of communities. It is, therefore, essential to employ advanced analytical methods to accurately identify and assess the water sources we rely on.

As public awareness grows, there are increasing concerns of unregulated contaminants such as PFAS in our drinking water. PFAS are an extensive class of compounds, numbering in the thousands, yet current analytical methods primarily focus on a limited set, leaving the majority unmeasured. Regulatory bodies like the US Environmental Protection Agency (USEPA) have established health advisory limits and proposed regulations to mitigate risks associated with PFAS exposure in drinking water.

The USEPA has set interim drinking water health advisory limits (HALs) for specific PFAS compounds, such as PFOA, PFOS, GenX, and PFBS, underscoring the urgency of monitoring and regulating these substances to minimize public exposure and associated risks.

The proposed National Primary Drinking Water Regulation by the EPA further highlights the importance of continuous assessment and regulation of PFAS. The inclusion of additional PFAS compounds in the proposed regulation emphasizes the need for improved analytical technologies to effectively detect and measure these substances at low concentrations.

With the goal of enhancing PFAS analysis workflows to meet these evolving regulations, Agilent has prepared a suite of helpful resources. Explore them below, free to download.

Gain ultimate sensitivity of PFAS in water

Find a method for the separation and detection of 28 PFAS chemicals in water samples using ultrahigh-performance liquid chromatography with tandem mass spectrometry.


PFAS – The problem that won’t go away

Download this infographic to explore the problem of PFAS. Discover facts and figures to enhance your understanding of the importance of PFAS testing and find effective analysis solutions.

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Is our water safe to drink?

Prof. Detlef Knappe, from North Carolina State University, shares his work analyzing contaminants in drinking water. Knappe outlines the analytical methods used to identify and assess contaminants, including PFAS, to understand routes of contamination and determine the safety of drinking water. Knappe also highlights how Agilent supported the development of these analytical methods.


Analyzing PFAS in wastewater is an essential consideration in preserving our environment and safeguarding living organisms from the escalating threat posed by these persistent compounds.

PFAS, due to their exceptional chemical stability and propensity for bioaccumulation, present a risk to the environment and its inhabitants. However, precisely detecting PFAS at trace levels is often challenging, given the interference from the complex environmental matrix and background contaminants.

Sensitive and robust analytical tools and methods are essential for accurate detection, risk assessment, regulatory compliance, efficient treatment, and informed decision-making concerning PFAS in wastewater. Current PFAS analysis technologies have made significant advancements in sensitivity, but challenges persist. The sensitivity required for PFAS analysis is exceptionally high due to their harmful effects even at very low concentration.

In response to this challenge, a comprehensive workflow has been devised for effective PFAS analysis in wastewater. This innovative approach builds on the EPA draft Method 1633, integrating additional PFAS variants in terms of size and functional groups. The workflow encompasses offline solid phase extraction (SPE), a crucial cleanup step, LC-MS/MS analysis, and automated reporting, culminating in a highly reliable solution for precisely analyzing targeted PFAS in complex matrices while maintaining robustness.

To further aid scientists and analysts in this critical pursuit, Agilent presents an advanced methodology for the separation and detection of 28 PFAS compounds in water samples. This comprehensive method leverages  state-of-the-art LC/MS technology, supported with software. This methodology covers all PFASs outlined in the ASTM 7979 method, utilizing the same sample preparation protocol. With a reporting limit of 10 parts per trillion (ppt, ng/L) or lower for most compounds, this technique offers a highly sensitive approach to precisely analyze PFAS in water samples.

Simple and fast analysis of PFAS in non-potable waters

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A comprehensive workflow for PFAS analysis in wastewater

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Data interpretation for wastewater

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The 6495D triple quadrupole LC/MS system is an ultrahigh-performance system built for research and testing labs investigating large batches of samples.

If you are under pressure to deliver meaningful results, the Agilent 6495 LC/TQ provides sensitivity without compromising robustness.  Built-in instrument intelligence of the 6495 LC/TQ enhances throughout and integrates comprehensive workflows to get your lab up and running fast, allowing even novice users to generate expert results.


The Revident LC/Q-TOF embodies the next generation of quadrupole time-of-flight instruments featuring truly innovative (electronic) instrument architecture, instrument intelligence, a novel, ultrafast detector, and an updated, robust calibrant delivery system.

The presence of PFAS in biofluids has garnered significant attention from the media, public, and government regulators. PFAS have come under intense scrutiny due to their persistent nature and inherent toxicity, demanding a thorough exploration of their presence and effects within the human body.

In the public eye and regulatory discussions, a majority of the focus centers on a limited set of PFAS, primarily PFOA and PFOS. However, the scope of conventional targeted methods is often restricted to measuring around 30 PFAS compounds, leaving a considerable gap in the assessment of these substances and their potential impact on human health.

To bridge this analytical gap and comprehend the comprehensive implications of PFAS loads on human health, there is a compelling need for non-targeted PFAS analysis. This approach is essential to broaden the coverage of PFAS measurement, encompassing the lesser-measured or unknown PFAS variants that are continually emerging with evolving manufacturing practices. Understanding the full spectrum of PFAS is critical for a comprehensive assessment of their impact on human health and the environment.

In response to this demand, Agilent has introduced FluoroMatch 3.0 – an innovative tool designed to elevate PFAS analysis. This version presents new developments and advancements in PFAS analysis methodologies. Explore its application to dried blood spots, providing a deeper understanding of PFAS exposure and shedding light on previously unexplored aspects of their presence in biofluids.

Automated PFAS non-targeted analysis and visualizations applied to mammalian biofluids

  • FluoroMatch software initiates its data analysis workflow by importing MS and MS/MS data collected through MS, MS/MS data-dependent (DDA), iterative exclusion MS/MS (IE-DDA), or targeted MS/MS modes from both individual and pooled samples, as well as blank samples

  • FluoroMatch employs sophisticated algorithms that encompass file conversion, blank filtering, feature annotation, and visualization

  • FluoroMatch software seamlessly imports data initially processed using Agilent’s Mass Profiler software or other peak picking software, enhancing versatility and integration in the analysis process

See how FluoroMatch can be used to classify mixtures, identify compounds, and determine unknowns.

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Improve your PFAS workflow

Explore Agilent's tips, tricks, resources, and research to learn how to:

  • Quickly set up robust PFAS testing with an easy-to-use eMethod that includes a step-by-step workflow guide and ready-to-run acquisition and quantitation methods

  • Analyze a wide range of PFAS compounds with a method to detect over 100 native and isotopically labeled PFAS compounds in drinking water and wastewater

  • Meet evolving PFAS testing needs with methods that include target compounds that are part of standard methods and regulatory lists, suchas US EPA, ASTM and ISO methods, European Union methods, Germany DIN methods, and Japan Drinking Water Quality Standards

  • How LC/TQ, GC/TQ, LC & GC-Q/TOF along with other techniques will be required to completely characterize PFAS in the future

How Weck Laboratories stays ahead in PFAS testing

Find out how Weck Laboratories is harnessing the capabilities of Agilent’s cutting-edge technology to revolutionize their PFAS analysis. Discover how this customer's experience with advanced solutions has streamlined their processes and enhanced their results, paving the way for more accurate and impactful environmental insights.

The PFAS lab of the future

Analyze multiple new classes of PFAS at the lowest levels in myriad environmental matrices while delivering rapid turnaround of highest quality results.

Targeted quantification and screening of PFAS compounds

Unravel the PFAS puzzle with comprehensive workflows

"Great product! It was recently installed at my undergraduate serving institution. The support from Agilent has been top tier!"

Josette Wilkes
Livingstone College