Quantitative live-cell analysis of human iPSC-derived neurons: Optimization of culture conditions and evaluation of cell health

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Quantitative live-cell analysis for optimization of culture conditions and evaluation of cell health in human induced pluripotent stem cell-derived neurons A major limitation in studying human diseases affecting the nervous system is the ability to culture, monitor and analyze neuronal cells that accurately represent human phenotypes of these disorders. The use of human induced pluripotent stem cell (hiPSC)-derived neurons has provided a new approach aimed at modeling neurological diseases. Monitoring neuronal morphology and cell health in long-term culture is critical for the characterization and evaluation of these novel model systems. Traditional approaches rely on endpoint assays and imaging techniques that require immunochemical staining, which provide limited real-time kinetic information. In this webinar, we highlight optimal culture conditions and demonstrate the ability of the IncuCyte S3® approach for real-time, long-term quantitative analysis of iPSC-derived neuronal cell health.