Neurobiological Markers of Cocaine Use Disorder
21 Jul 2015

Cocaine use disorder is characterized by chronic cycles of abstinence and relapse. Prevention and treatment of cocaine use disorder could be greatly advanced by a more comprehensive understanding of its neurobiological underpinnings. Alterations in the serotonin and glutamate neurotransmitter receptor systems in the brain are putative molecular mechanisms, which may be involved in the propensity to relapse to cocaine use. We optimized and implemented a quantifiable and reproducible automated Simple Western assay in Wes™ to examine serotonin and glutamate receptor expression, as well as protein:protein interactions from rodent brain samples.

In this webinar, Dr. Noelle Anastasio, Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch; and Sarah Swinford-Jackson, Graduate Assistant, Center for Addiction Research, University of Texas Medical Branch, will discuss:

  • Validation of commercially available antibodies in vitro and ex vivo for serotonin receptors using the Wes system
  • Interrogation of serotonin receptor expression in preclinical models of cocaine use disorder
  • Investigation of glutamate receptor expression in preclinical models of cocaine use disorder
  • Exploration of serotonin receptor and glutamate receptor protein:protein interactions

ProteinSimple (formerly Cell Biosciences)

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