Measurements of Protein Refolding using the UNit
20 February 2017
This application note describes how the UNit can be used to monitor the protein denaturation and refolding. The heating/cooling plate included in the UNit enables a range of software managed heating and cooling profiles to be performed, whilst simultaneously assessing protein structure via intrinsic fluorescence measurement and aggregation state using static light scattering (SLS). Using the UNit enables the use of small sample volumes, time efficient acquisition of multiple data sets and an economical method for screening a range of conditions during one experiment.