High Resolution and Rapid Peptide Mapping of Monoclonal Antibody Using an Agilent 1290 Infinity UHPLC and an Agilent 6550 iFunnel Q-TOF LC/MS System
9 October 2015

Peptide mapping is the common analytical technique for characterizing biologic therapeutic proteins. The protein is first digested into smaller peptides enzymatically using one or two proteases. The peptide mixture is typically separated by reversed-phase chromatography and detected by UV or mass spectrometry. This application note describes how peptide mapping of an IgG (1) therapeutic monoclonal antibody (mAb) was performed using a novel superficially porous peptide mapping column coupled to an Agilent 6550 iFunnel Q-TOF LC/MS System.