Characterization of Monoclonal Antibodies Using Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)
23 Jan 2015
This application note demonstrates the utility of capillary electrophoresis/mass spectrometry (CE/MS) for the analysis of intact monoclonal antibodies (mAbs). The Agilent 7100 CE System coupled to an Agilent 6530 Q-TOF LC/MS was used to study the intact and reduced forms of mAbs. The combination of mass measurements obtained by CE/MS and the data processing capabilities of Agilent MassHunter and BioConfirm Software enables the identification of heterogeneity in an intact mAb and its fragments.