Precise Determination of Protein Molecular Weight Using the Agilent 1260 Infinity Multi-Detector GPC / SEC System
21 May 2015

This application note shows the expansion of the Agilent 1260 Infinity Bio-inert Quaternary LC System with the Agilent 1260 Infinity Multi-Detector GPC/SEC System for the determination of molecular weight, size, and relative concentration of protein samples after size exclusion chromatography. Accurate identification and quantification of proteins ranging from 11 kDa to 670 kDa was obtained using a tetra detection setup with concentration detectors, dual angle light scattering, and viscometer.