Commitment to Covalency: Kinetics of Irreversible Inhibitors with a Regenerable Streptavidin Sensor on the Pioneer FE System
27 June 2017

The factors contributing to the potency of irreversible inhibition are poorly understood. This application note details Dr. Schwartz’s method for combining a reversible streptavidin-biotin capture method with the real-time direct observation of binding/reaction. It utilizes the Pioneer FE system from ForteBio and combines this with biochemical data to provide a full assessment of the inhibitor’s protein association, dissociation and reactivity. This ‘best of both worlds’ strategy provides the benefits of both biochemical and biophysical methodologies.