Bead Mill “Beadless” Protein Extraction from Soft Tissues with the Bead Ruptor 24
17 Oct 2014

The Bead Ruptor 24 Bead Mill Homogenizer vigorously shakes tubes filled with bead material to disrupt samples such as soft tissues. The impact between the beads and the sample reduces sample particle size and creates a homogenous mixture. While bead milling is an efficient method of homogenization, for some applications, the presence of the bead media within the sample can be a drawback. Heat generation can be problematic when extracting volatile or heat sensitive samples such as proteins or RNA. This application note evaluates the feasibility of a bead mill based “beadless” homogenization for protein extraction from soft tissues. Protein extraction efficiency is analyzed following a homogenization procedure in which tissues are homogenized on the Bead Ruptor 24 in tubes without beads present.

Show    per page
1 of 8     Page  1 2 3  ... Next 
How to Facilitate Filtration of Small Volumes for HPLC and UHPLC Sample Prep MS syringe filters, syringe filtration17 Oct 2014Read
Development of Low Extractable Syringe Filters for Liquid Chromatography/Mass Spectrometry Applications MS syringe filters, syringe filtration15 Oct 2014Read
Lysate Clearance Using the AcroPrep™ Advance Filter Plate molecular biology13 Oct 2014Read
Double Stranded DNA Quantitation Using Promega QuantiFluor® Dyes automation, DNA2 Oct 2014Read
Using UPLC-MS/MS for Workplace Drug Testing drugs of abuse29 Sep 2014Read
Structural Changes in Polymers by Means of Cryogenic Grinding temperature control, milling, polymers24 Sep 2014Read
Automated NGS Library Prep: Ligation Efficiency of NxSeq® Sample Preparation Using the Gilson PIPETMAX® 268 library preparation, NGS22 Sep 2014Read
Extraction of Labile Enzymes from Gram-Positive Bacteria with the FastPrep System bacteria, enzymes19 Sep 2014Read
Compound Extraction from Whole Lung of Rats with Precellys®24 Dual – 7 ml Vial sample homogenizer, homogenizing, animal models19 Sep 2014Read
Protein extraction from Arabidopsis with Minilys plant science19 Sep 2014Read

Popular tags in SelectScience

Buying guides