Bead Mill “Beadless” Protein Extraction from Soft Tissues with the Bead Ruptor 24
17 Oct 2014
The Bead Ruptor 24 Bead Mill Homogenizer vigorously shakes tubes filled with bead material to disrupt samples such as soft tissues. The impact between the beads and the sample reduces sample particle size and creates a homogenous mixture. While bead milling is an efficient method of homogenization, for some applications, the presence of the bead media within the sample can be a drawback. Heat generation can be problematic when extracting volatile or heat sensitive samples such as proteins or RNA. This application note evaluates the feasibility of a bead mill based “beadless” homogenization for protein extraction from soft tissues. Protein extraction efficiency is analyzed following a homogenization procedure in which tissues are homogenized on the Bead Ruptor 24 in tubes without beads present.