A Fully Automated Primary Screening System for the Discovery of Therapeutic Antibodies Directly from B Cells
26 August 2015
For a therapeutic antibody to succeed, it must meet a range of potency, stability, and specificity criteria. In this case study, Tickle, S. et al from UCB Pharma describe the successful design, construction and commissioning of a bespoke automated screening platform, incorporating mirrorball, and how it can be used for screen antibodies against two specific targets. This automated platform can combine high throughput B-cell culture screening with the identification and isolation of single, antigen-specific IgG-secreting B-cells through a proprietary technique called ‘fluorescent foci’.