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Arizona State University
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"The XTT Kit is easy to use and has a shorter incubation time compared to the MTT assay. The kit is sensitive and produces accurate results. It is great value for the price and I would highly recommend it."
XTT Cell Proliferation Assay Kit procedure avoids radioactivity, allows for rapid determination in microplates, and gives reproducible and sensitive results.
Measurement of cell viability and proliferation comprise the underlying basis for numerous in vitro assays directed towards the quantitation of a cell population's response to external factors. Cell proliferation assays have utilized the uptake of radiolabeled thymidine into cellular DNA, however, this method is time consuming and involves the use of hazardous materials.
The use of tetrazolium salts, including XTT (2,3-Bis(2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium-5-carbox-anilide), to assay cell proliferation, cell viability, and/or cytotoxicity is a wide-spread, established practice.
Cleavage of the tetrazolium salt to formazan occurs via the succinate-tetrazolium reductase system in the mitochondria of metabolically active cells. The reaction is attributed mainly to mitochondrial enzymes and electron carriers, but a number of other non-mitochondrial enzymes have been implicated.
XTT, a yellow tetrazolium salt, is cleaved to a soluble orange formazan dye, which can be measured by absorbance at 490 (or 450) nm in a microplate reader. Efficient reduction of XTT requires an electron coupling reagent.
Trevigen’s TACS® XTT kit includes XTT and an electron coupling reagent for a more rapid, convenient and simple assay.