VECTASHIELD® Mounting Medium with DAPI by Vector Laboratories Inc.

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VECTASHIELD® Mounting Medium with DAPI by Vector Laboratories Inc. product image
VECTASHIELD® Mounting Medium with DAPI
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Average Rating: 4.5
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money

Rating: 4.3

  • Application Area: ICC/IHC

"The Vectashield with DAPI is an incredibly useful convenient nuclear counterstain. We have successfully used it for a number of cells and tissue types in our laboratory. It is easily applied with a dropper and doesn't require any additional washing steps. "

Review date: 11 Nov 2015 | VECTASHIELD® Mounting Medium with DAPI
  • Status:

    Advanced Reviewer

  • Member since: 2014

  • Organization: Universidade Federal de Minas Gerais

  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Nucleus staining

"VECTASHIELD® Mounting Medium with DAPI stains nucleus very well and you do not need to incubate DAPI for one hour and then use one mounting media. This product is very stable and simple to use. Once you prepare the cover slips directly with it, you will save one protocol step less, which decreases your error chances. DAPI signal is strong enough and the slides can be stored at 4ºC for subsequent analysis. VECTASHIELD® Mounting Medium with DAPI is also stored at 4ºC and can be used for long time."

Review date: 08 Dec 2014 | VECTASHIELD® Mounting Medium with DAPI
VECTASHIELD with DAPI - VECTASHIELD® Mounting Medium is a unique, stable formula for preserving fluorescence.

VECTASHIELD Mounting Medium with DAPI prevents rapid photobleaching of fluorescein, rhodamine, Texas Red®, and other fluorescent reagents such as Cy3, Cy5, Alexa Fluor® 488, Alexa Fluor® 594, and DyLight™ dyes.* Sections mounted in VECTASHIELD® Mounting Medium retain fluorescence during prolonged storage.

VECTASHIELD DAPI Mounting Medium is supplied in a bottle fitted with a cone-seal cap. A cap fitted with a drop dispenser pipet is also included. The cone-seal cap can be replaced with the drop dispenser cap for routine use and during storage. Although the pipet bulb is made of inert materials, it is suggested that once the drop dispenser cap has been secured on the bottle, the bottle should be stored in an upright position.

To mount tissues or cells on a slide, dispense one drop of VECTASHIELD with DAPI Mounting Medium onto the section. Then coverslip and allow VECTASHIELD® Mounting Medium to disperse over the entire section. Applying an excess of mounting medium is not recommended. The special pipet supplied with the product is designed to deliver small drop volumes of approximately 25 μl.