Trypsin In-Gel Kit

Trypsin In-Gel Kit

Sigma-Aldrich

Trypsin Profile IGD Kit Fast, Efficient, Complete Digestion, from PAGE to MALDI. The Trypsin IGD Kit (PP0100) enables fast, efficient, and complete in-gel tryptic digest of up to 100 excised prot...read more

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Description

 

Trypsin Profile IGD Kit
Fast, Efficient, Complete Digestion, from PAGE to MALDI.

The Trypsin IGD Kit (PP0100) enables fast, efficient, and complete in-gel tryptic digest of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS and require no additional preparation. Because the Trypsin IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra. 
The Trypsin IGD Kit contains Proteomics Grade Trypsin and is optimized for polyacrylamide gels stained with Coomassie, SYPRO® Orange or Ruby dyes. If silver staining is used, ProteoSilver™ Plus silver staining kit is recommended (Product Code PROT-SIL2).

Features & Benefits:

Includes highly purified Proteomics Grade Trypsin. Trypsin displays only minimal autolytic activity and no chymotryptic activity, which simplifies resulting peptide analysis.

Detailed protocol. Easy to follow protocol simplifies the process.

Works with Coomassie, SYPRO Orange, SYPRO Ruby or ProteoSilver Stained gels. Allows user a choice of staining methods.

Resulting samples are suitable for analysis by MALDI-MS. No further processing needed, saves time.

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