Transcriptor High Fidelity cDNA Synthesis Kit


Roche Applied Science - a member of the Roche Group

The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelit...read more

Request Pricing





Receive your quote directly from Roche Applied Science - a member of the Roche Group for Transcriptor High Fidelity cDNA Synthesis Kit

Description

 

The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications:

  • Cloning genes of interest
  • Sequencing transcriptomes
  • Generating cDNA libraries with large and full length inserts

As the kit is also tested with the LightCycler® Instruments and other real-time PCR Instruments, the product is also ideal for quantitative RT-PCR applications that require high fidelity.
The kit contains all components required for synthesizing cDNA suitable for direct use in qualitative RT-PCR with conventional thermal cyclers or quantitative RT-PCR on real-time PCR instruments. The 50-reaction pack size also includes 10 control reactions (control RNA and control primer mix).

Benefits
Increase accuracy during reverse transcription reactions.

  • Benefit from an optimized enzyme blend with a 7-fold higher fidelity compared to other commonly used reverse transcriptases.
  • Trust true-fidelity data, as determined by the sequencing of amplified fragments with the Genome Sequencer 20 System.

Combine accuracy with high sensitivity, high yield, and full-length transcripts.

  • Generate full-length transcripts up to 14 kb with the anchored-oligo (dT)18 primers included in the kit.
  • Obtain excellent yields.
  • Detect low-copy number templates; reverse transcription from as little as 1 ng template RNA is possible.
  • Transcribe a variety of templates, even the most difficult (e.g. GC-rich RNAs with high secondary structure) through reverse transcription at temperatures up to 55°C.

Get results faster.

  • Reduce reverse transcription reaction time to as little as 10 minutes.

Feedback

Request info

To:Roche Applied Science - a member of the Roche Group
From:
Please contact me / call me with the Transcriptor High Fidelity cDNA Synthesis Kit...,

I am looking to purchase in /

.
About you

Write a review

Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad air (All reviews published will be entered into the next drawing on May 30th 2014).

  
Your Email

 



Ease of use

After sales services

Value for money

WATCH:
The benefits of becoming a reviewer
Share your opinions...()
HINTS & TIPS
Give reasons for the star ratings you have awarded for each area.
Would you recommend this to a colleague?
What would you like to have known about equipment and consumables before you purchased?



Why make an enquiry with SelectScience?

  • EASY It’s quick and simple to do
  • FAST Your enquiry will be delivered straight to the manufacturer
  • FREE You’re under no obligation
  • SECURE We only pass your details on to trusted suppliers at your request
  • SAVE TIME Submit your details once and make multiple enquiries
SelectScience Trusted Banner