Cell Line Description: The ToxReporter HRE Cell Line contains a reporter gene under transcriptional control of the Hypoxia Response Element (HRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the HRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can then be quantified using the ToxReporter ELISA kit. This cell line has been optimized for assay performance including plate seeding number, stimuli concentration for optimal response and stimulation time. Validations for assay performance include EC50 and Z’. Pathway Description: Virtually all toxic responses are preceded by the transcriptional activation of stress respon...Read more
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