Thermo Scientific Fluoroskan Ascent

Thermo Scientific Fluoroskan Ascent
 4.3/5.0 (1 review)

Thermo Fisher Scientific

Thermo Fluoroskan Ascent, a dedicated microplate fluorometer - High sensitivity for a wide variety of fluorometric applications. The Thermo Fluoroskan Ascent features compatibility with 1- to more

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Julie Hjelmhaug


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Member since 2012

Job Title:
Neuroscience / Neurology
Work Field:
Academia / University
Marquette University
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  • 5 out of 5EASE OF USE
  • 4 out of 5VALUE FOR MONEY

Review date: 29 Jan 2013

Application Area: glutathione assays
Thermo Scientific Fluoroskan Ascent

"The Fluoroskan is very easy to use, and fast. Just choose your wavelengths, plates, and read. The hard part is your sample prep. Our sales person was quite nice, and accommodating. We've had the instrument for several years, and have had no problems with it, so we haven't had to have any service calls yet. It really is a low maintenance instrument."



Thermo Fluoroskan Ascent, a dedicated microplate fluorometer - High sensitivity for a wide variety of fluorometric applications.

The Thermo Fluoroskan Ascent features compatibility with 1- to 384- well plate formats and PCR plates, up to three on-board dispensers, an incubator and a shaker. 

Application areas of Fluoroskan Ascent

Calcium flux assays, Reporter gene assays, Immunoassays, FRET assays, Hybridization assays
Cell proliferation
Enzyme activity
Multi-drug resistance
Cell adhesion
Bacterial quantitation
Quantitation of PCR products
Molecular beacon assays
DNA quantitation

Advantages of the Thermo Fluoroskan Ascent include:

State-of-the-art optical system for unrivaled sensitivity
Comprised of a mirror and lenses, the advanced optical system of Fluoroskan Ascent produces a highly focused light beam. The sharp focus prevents crosstalk and ensures accurate readings.

Up to three dispensers for flash and rapid kinetic applications
For easy and accurate reagent addition, up to three dispensers can be fitted on board Fluoroskan Ascent. Dispensing volumes can be adjusted in 1µl increments over a volume range of 1-1000 µl. 

Flexible plate formats, from 1 to 384, enable reading in primary wells
Designed for superior flexibility, Fluoroskan Ascent reads any microplate format, including 1-96 and 384-well plates. The format is simple to select from a database covering the most common plate types.

The Thermo Fluoroskan Ascent reads from above or below the plate
The easy-to-switch optical unit reads from above or below the plate. For cell biological applications, reading from below the plate allows closer proximity to cells and greater sensitivity.

On-board incubation and orbital shaking
An incubator is available for cell biology, enzyme assays and other applications requiring precise temperature control. Built-in orbital shaking of the Fluoroskan Ascent, another advanced feature, speeds up reaction times and ensures effective mixing.

The Fluoroskan Ascent is Completely robot compatible
Robotic integration is simple and effective with the Fluoroskan Ascent. In addition, wall edges of the carrier have been beveled to aid the plate to slide down to the carrier.


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